MIDI rolls don't, by themselves, make any noise or even inherently define any note frequencies at all.
The frequency of the sound produced by a given synthesizer when it receives any particular MIDI note number is up to the synthesizer. This is part of the point of the MIDI tuning system. The synthesizer and/or the tuning system may very much care about keys and accidentals.
The frequency of the sound produced by a given synthesizer when it receives any particular MIDI note number is up to the synthesizer. This is part of the point of the MIDI tuning system. The synthesizer and/or the tuning system may very much care about keys and accidentals.