That's one of the possibilities, but if we're talking about those kinds of hypotheticals, allow me another one. If you recall, one of the themes of the later Dune books was that humanity was fleeing from the frontiers (The Scattering) back into the heartland from some unknown but extremely powerful enemy. Perhaps those were simply the neighboring species who did not have a Butlerian Jihad of their own?
I don't recall anything indicating that in Frank Herbert's original series. And Brian Herbert's "Omnius" nonsense seems to have very little to do with what Frank actually had in mind wrt Butlerian Jihad etc, so I don't consider it canonical.
It describes humans hiding from "seeker machines", which does indeed imply some kind of AI. But whether that AI is the enemy or merely one of its tools is left unclear.
