Yeah for sure. I've noticed this technique before in some great comedians - for example Andy Kaufman and Will Ferrell. In fact - that was what really got me about Kaufman's bits - the utter lack of blinking. I love how it makes the character seem possessed by some personality outside their own self.
So of course when that character doesn't blink it genuinely f*cks with out sense of balance in our communication - In the case of comedy, it's hilarious. Maybe in the case of a physical confrontation, it would be threatening instead..
I also think that when we are in conversation with other people, we innately calculate when to blink based on when they blink. http://www.telegraph.co.uk/science/science-news/5925932/Huma...
So of course when that character doesn't blink it genuinely f*cks with out sense of balance in our communication - In the case of comedy, it's hilarious. Maybe in the case of a physical confrontation, it would be threatening instead..